Firstly, we generated a dot plot (Figure 3A) according to expression level of NUSAP1 in 52 normal samples and 115 HCC samples by GraphPad Prism; the result showed that expression level of NUSAP1 in tumor samples was significantly higher than that in normal samples (p < 0.0001). This evidence concerns the gene NUSAP1 and hepatocellular carcinoma.