Firstly, we did survival analysis, clinical correlation analysis, independent prognostic analysis, and GSEA enrichment analysis of NUSAP1 by HCC patients in GSE76427 dataset, ICGC database, and TCGA database; we verified that the higher expression of NUSAP1 was closely related with poorer prognosis; it was also an independent prognostic factor in HCC. Here, NUSAP1 is linked to hepatocellular carcinoma.