Firstly, we drew a dot plot (Figure 4A) according to NUSAP1 expression level in 202 normal samples and 243 HCC samples using GraphPad Prism; the result showed expression level of NUSAP1 in tumor samples was significantly higher than that in normal samples (p < 0.0001), which was same as the result we got by GSE76427 dataset. This evidence concerns the gene NUSAP1 and neoplasm.