To mimic the postsurgical standard of care treatment, we subjected GBO samples from patient whose GBM tissues expressed high CD68 and SPP1 (Supplementary Figure S13B) to a single exposure conditioned medium from HSPA7-knockdown GSCs or with PD1 inhibitor (5 μM) treatment for 5 days. The gene discussed is SPP1; the disease is glioblastoma.