Additionally, both Klotho variables in our study were significant predictors of subclinical atherosclerosis in a full adjusted model that included important cofounders such as age, HT, smoking, eGFR, DM, dyslipidemia, macroalbuminuria, and the serum and PBCs expression levels of the cytokines IL6, TNFα, and IL10. The gene discussed is TNF; the disease is diabetes mellitus.