SLC18A3 and hyperinsulinemic hypoglycemia, familial, 4: Both groups of mice submitted to the elastase protocol (WT-PPE and VAChT KDHOM-PPE) showed reduction in tissue elastance when compared to control animals that received saline (WT-SAL and VAChT KDHOM-SAL, P < 0.05 for both comparisons)], but the effect was similar in both genotypes, suggesting that cholinergic deficiency does not make tissue elastance worse (Fig. 1A).