To sum up, in order to overcome the challenge of cancer resistance, we design a CRISPR/Cas9 vector system that can delete a total of 563 bp of HPV18 E6 and E7 genes, effectively eliminating viral genes integrated into the host genome, simultaneously reactivate the p53 and pRB tumor suppressor pathways, resulting in cervical cancer cell death. Here, TP53 is linked to cervical carcinoma.