IL25 and obesity due to melanocortin 4 receptor deficiency: However, IL-25 injection could reduce body weight gain (S3C Fig) and eWAT mass (S3D Fig), lower liver weight (S3E Fig) in mice with DIO that were injected with clodronate-loaded liposomes, suggesting that macrophages are not necessary for all of the anti-obesity effects activated by IL-25.