After vaccination with H1-DDA/TDB, both IFN-γ+TNF+IL-2+ triple-positive and IFN-γ−TNF+IL-2+ double-positive multifunctional T cells can be detected > 1 year post-vaccination in spleens and peripheral blood and accumulate in the lungs of vaccinated mice after infection with Mtb [35]. Here, TNF is linked to infection.