Additionally, our data showed that IGFBP1 and ATF4 were both induced as the ISR-target gene in CBD-treated HCC cells (Figure 5C), consistent with data from the literature that the ISR-dependent ATF4 induction may be upstream of IGFBP1 (Marchand et al., 2006; Magne et al., 2011). This evidence concerns the gene IGFBP1 and hepatocellular carcinoma.