The relative expression of lncRNA NKX3-1 in glioma cell lines A172, U251, U373 and U87 was significantly higher than that in HEB cell lines (P <0.05), and the relative expression of lncRNA NKX3-1 in U87 cell line was the highest compared with that in HEB cell line (P <0.001), as shown in Figure 4. The gene discussed is NKX3-1; the disease is glioma.