Surprisingly, the reduced Akt and p-Akt expression level efficiently restrained CSC-targeted anticancer activity of TDB as evidenced with no significant alteration of tumor-initiating cells (Figure 7(d)), number of formed colonies under attachment condition (Figure 7(f)), and relative CSC spheroid size in siAkt-transfected H460 cells culture with 5 μM TDB (Figure 7(h)). The gene discussed is AKT1; the disease is neoplasm.