After 48 h of transfection, the significant downregulation of Akt and p-Akt protein levels was demonstrated via Western blot analysis in lung cancer H460 cells transfected with siAkt compared with both wild-type (WT) cells and si-mismatch control- (siCtrl-) transfected cells (Figure 7(a)). This evidence concerns the gene AKT1 and lung cancer.