Finally, we investigated whether KFMT-induced defecation delay effects are accompanied by alterations in the molecular regulators for constipation, including AQP3, C-kit, and 5-HT, because their roles are associated with the regulation of water content, proliferation of interstitial cells of Cajal, and gastrointestinal mobility during constipation. Here, AQP3 is linked to Constipation.