In conclusion, we determined that KX-01 was able to suppress cellular proliferation, decrease the expression of HIF-1α, p-Src, α-SMA, collagen I, and collagen III, and lower the p-STAT3/STAT3 ratio in vitro and in vivo, suggesting that this compound may alleviate experimental pulmonary fibrosis by modulating the p-SRC/p-STAT3 signaling pathways. This evidence concerns the gene HIF1A and pulmonary fibrosis.