PITX2 and colorectal carcinoma: To evaluate the effect of RGS on CRC cell viability, 7 CRC cell lines (SW48, Caco-2, DLD1, HCT116, LOVO, SW620, and SW480) with a different RAS mutational status were treated with RGS at concentrations ranging from 0 to 1,000 nM or 0 to 20 μM for 96 h or 48 h, respectively.