We also treated CCA cells with the transcription inhibitor, actinomycin D (Act D)25,31, and then determined the RNA stability of CTNNB1 transcripts in the control and METTL3-, METTL14-, or IGF2BP2-knockdown cells (Supplementary Figure S3A, S5D). This evidence concerns the gene IGF2BP2 and cholangiocarcinoma.