An m6A RNA immunoprecipitation assay showed that the lower enrichment of m6A modification was associated with some stemness-regulated genes, including CTNNB1, in METTL3- and METTL14-silenced CCA cells, which is consistent with the data in the GSE90642 data set based on a study of HepG2 cells31 (Figure 4A, B and Supplementary Figure S5A). This evidence concerns the gene CTNNB1 and cholangiocarcinoma.