To clarify the function of MCM3AP-AS1 in NSCLC progression, A549 cells were co-transfected with MCM3AP-AS1 overexpression plasmid and miR-195-5p mimics; H226 cells were co-transfected with sh-MCM3AP-AS1#1 and miR-195-5p inhibitors, and qRT-PCR showed that the transfection was successful (Figure 3a). This evidence concerns the gene MCM3AP and non-small cell lung carcinoma.