Transcriptome (RNA-Seq) analysis of the cytoplasmic poly(A)-tailed transcripts (total fraction) and poly(A)-tailed transcripts associated with polysomes (polysomal fraction) was carried out at 6 and 10 h p.i., corresponding to time points prior to and during the MNV-induced phosphorylation of eIF2α by GCN2 (23) in RAW264.7 cells infected at a multiplicity of infection (MOI) of 10 with matching controls using UV-inactivated virus—MNV(UVi) (Fig. 2A). This evidence concerns the gene EIF2AK4 and infection.