To explored the mechanism of ADAM10 regulating the CXCL16 function in GC, we established ADAM10 knockdown cell lines in MGC803 and SGC7901 respectively, as the ADAM10 knocking down efficiency showed in Fig. 5A. To investigate whether ADAM10 involved in CXCL16 regulation, we compared the proliferation, colony formation and migration ability of the cells overexpressed with CXCL16 in ADAM10 known down cell lines (Fig. 5B-D). This evidence concerns the gene CXCL16 and gastric cancer.