To evaluate the fundamental role of ULK1/2 on PKM2 expression in vivo, we examined PKM2 expression using immunohistochemistry (IHC) in subcutaneous tumor xenografts arised from nude mice that were inoculated with S-1 cells bearing ULK1, ULK2 or ULK1/2 shRNA. The gene discussed is ULK1; the disease is neoplasm.