To confirm the above findings, migration and invasion assays were also conducted in CRC cells with RNF141 overexpressing, and the results showed that RNF141 overexpression exerted promotive effects on cell migration and invasion that LV-RNF141 group showed a significant increase of wound closure compared to LV-NC group (Fig. s3A). Here, RNF141 is linked to colorectal carcinoma.