RNF141 and colorectal carcinoma: Then LV-NC or LV-RNF141 transfected CRC cells were treated with palmostatin B. IF assay showed that the treatment of palmostatin B caused a loss of KRAS membrane localization in HCT116 cell transfected with LV-NC or LV-RNF141, compared with DMSO treatment (Fig. 8E).