Subsequently, to further elucidate the underlying mechanism by which RNF141 exerted its functions in CRC, we combined IP and LC–MS/MS to screen out the target proteins that interacted with RNF141, and identified more than 30 cancer-related proteins in total 143 proteins that might have interaction with RNF141, including tumor-suppressor factors, such as KLF4, and oncogenic factors, such as KRAS, YY1, LYPLA1, and so on. This evidence concerns the gene KLF4 and colorectal carcinoma.