As a result, Co-IF assay showed that KRAS co-localized with RNF141 and predominantly accumulated on the plasma membrane (Fig. 6C, yellow signal), To further determinate the endogenous interaction between RNF141 and KRAS, BiFC assay was carried out in CRC cells, and the results provided further support that there was indeed direct interaction between RNF141 and KRAS in vivo (Fig. 6D, Fig. s5A). Here, RNF141 is linked to colorectal carcinoma.