We further assessed the protein levels of NSUN2 in ESCC and observed that NSUN2 protein was expressed at significantly higher levels in ESCC tumors than in paired normal tissues by western blotting (n = 10; Fig. 1E) and by immunohistochemical staining (IHC) (n = 59; Fig. 1F, G; Supplementary Table 4). The gene discussed is NSUN2; the disease is esophageal squamous cell carcinoma.