We then performed in silico analysis using five publicly available databases (ChIPBase, GTRD, AnimalTFDB, JASPAR and hTFtarget) to search for cis-element(s) in the NSUN2 promoter region (from −1000 bp to the transcription start site) and also looked at a public microarray dataset of 179 paired ESCC (GSE53625) to search for the transcription factors (TFs) positively correlated with NSUN2 levels (r > 0.3, P < 0.05). Here, NSUN2 is linked to esophageal squamous cell carcinoma.