In this study, we found that overexpression of NSUN2 in ESCC was not caused by genomic changes as indicated by no significant alterations of mutations, CNV and DNA methylation status at NSUN2. Integrated analysis suggests that E2F1 was a potential regulator of NSUN2. E2F1 is a well-known TF that has been shown to be aberrant expressed in ESCC tumors [40]. Here, NSUN2 is linked to esophageal squamous cell carcinoma.