Highly expressed ANLN-210 mRNA delivered to tumor-associated macrophages (TAMs) via SCC-9 cells secreted exosomes, causing activation of macrophages polarized to M2 phenotype via the canonical PI3K/Akt signaling pathway, thus promoting tumor growth and metastasis of HNSCC. The gene discussed is AKT1; the disease is head and neck squamous cell carcinoma.