To further evaluate the role of the two splicing isoforms of ANLN in tumor development, we co-injected ANLN-sgRNA SCC-9 cells transfected with GFP-ANLN-201 or GFP-ANLN-210 or control, and macrophages treated with ANLN-210 mRNA-rich exosomes into the nude mice. Here, ANLN is linked to neoplasm.