ACTA1 and interstitial lung disease: In fibrocytes isolated from Scl70+ILD+ patients, nintedanib 0.1 μM and 1 μM significantly downregulated the gene expression of αSMA, S100A4, and FN compared to untreated cells after 3 h of treatment (p < 0.05 for both concentrations relative to all molecules) (Fig. 4A).