Indeed, Western blotting with an antibody raised against the N-terminal region of FGFR2 detected a band at approximately 200 kDa, a lower mobility than endogenous FGFR2 in FGFR2-amplified MFM-223 breast cancer cells and the PDX HCI-016 (140–150 kDa) (Fig. 3e). Here, FGFR2 is linked to breast carcinoma.