Tumor grade III was strongly associated with HGF positivity [53% vs. 9%; RFD = + 41.11%, 95% CI (36.68, 45.55)] Higher breast cancer stage was also associated with increased prevalence of HGF positivity when compared to stage I [36% for stage II vs. 24%; RFD = + 6.16%, 95% CI (0.21, 12.11), and 37% for stage III/IV vs. 24%, RFD = + 7.89%, 95% CI (3.54, 12.22)]; however, after adjustment for multiple comparisons, associations with HGF and stage III/IV were no longer statistically significant (FDR p value = 0.068). The gene discussed is HGF; the disease is breast cancer.