The objective of this study was to determine the capacity of various inflammatory mediators (LPS, PMA and A23187) to induce NETs, Ang1 and calprotectin synthesis and release, and if Ang1 can bind to NETs, using calprotectin as a positive control, from neutrophils of patients with stable or decompensated HF with or w/o T2DM compared with healthy control (HC) volunteers. Here, ANGPT1 is linked to type 2 diabetes mellitus.