Here, the tree stem encompassed several well-known mutations inlung cancer such as STK11 (prognostic marker, aids drug resistance withfusion partner LKB1, [22, 23], PTPN13 (tumor suppressor inlung adenocarcinoma) [24], NOVA1(promotes telomerase activity in NSCLC) [25],ANXA2 (influential on lung cancer cell survival, apoptosis, as well asa construct of EGFR- fusion gene) [26–28], RYR2 (associated with high NSCLC mutation burden inconjunction with exposure to high air pollution) and KLF4 (regulates lungcancer initiation) [29]. Here, KLF4 is linked to regulation of cell cycle.