Knockdown of homeobox B8 dramatically repressed the migration and invasion of osteosarcoma cells by preventing the activation of Wnt signaling pathway.33 A 2017 study34 has shown that Wnt signaling pathway activation may downregulate the expression of Beclin 1 and rescue chemotherapy drug resistance in osteosarcoma. This evidence concerns the gene HOXB8 and osteosarcoma.