To determine whether GAPDH progressively accumulates in CSF of AD patients, we studied samples from first cohort of patients with Mild Cognitive Impairment (MCI) (n = 22), as well as with mild (n = 41), moderate (n = 49), and severe (n = 51) AD, stratified based on Mini-Mental State Examination (MMSE) scores [20] (Supplementary Table 1). Here, GAPDH is linked to Alzheimer disease.