First, we found the expression levels of four novel hub genes (CENPL, ISG20L2, MRPL3, and LSM4) were significantly higher in the subjects aged ≤ 51 years, and high expression of CENPL, ISG20L2, MRPL3, and LSM4 were associated with lymph node metastasis and higher SBR grade (P < 0.05, Fig. 3a−c). This evidence concerns the gene LSM4 and metastatic malignant neoplasm in the lymph nodes.