ABCB1 and hepatocellular carcinoma: PAK5/WT-transfected and PAK5/S573N-transfected cells also hold a higher efflux of Rh123 fluorescence when compared with the transfection of PAK5/K478M and PAK5/Vector, which indicated that PAK5 was likely to augment the ABCB1 expression in HCC cell lines (Fig. 5B).