To detect the correlation of PAK5 and HCC sorafenib resistance, we used PAK5/S573N (constitutively active) plasmid and PAK5/K478M (constitutively inactive) plasmid and carried out four groups that were transfected with them, correspondingly, in Hep3B and HepG2 cell lines. Here, PAK5 is linked to hepatocellular carcinoma.