However, we obtained an increase in the amount of ligation products over the time of reaction incubation in the presence of the cancer-associated (P161L, R194W, R280H, R399Q, Y576S) and cerebellar ataxia-related K431N XRCC1 variants (Figs. S10, A–C and S11). This evidence concerns the gene XRCC1 and aceruloplasminemia.