We implemented this assay in a high‐content, 384‐well microplate format using HEK293T cells, which are not susceptible to viral infection unless transfected with ACE2 and TMPRSS2, and Calu‐3 lung cancer cells, which possess the replete machinery for spike‐mediated viral infection (Hoffmann et al, 2020a). Here, TMPRSS2 is linked to viral infectious disease.