To prove the universal inhibition role of miR-219-5p on different type of breast cancer cells, we performed migration and invasion assays by using two additional breast cancer cell lines, MCF-7, that is estrogen and progesterone receptor positive and HER2 negative, and BT474, that expresses estrogen and progesterone receptor plus HER2 protein. The gene discussed is PGR; the disease is breast carcinoma.