Consistent with the result of Western blot, IF showed reduced expression of E-cadherin and enhanced expression of vimentin in HCC cells after marvelD3 knockdown (Figure 3(e)), as well reduced expression of vimentin and enhanced expression of E-cadherin in marvelD3-expression HCC cells (Figure 3(f)). This evidence concerns the gene CDH1 and hepatocellular carcinoma.