We used the bioinformatic analysis to predict the possible lncRNA PVT1 targeting miRNAs and the miRNAs that might be aimed at regulating KLF5 function, and we discovered that one of the miRNAs, namely, miR186, was found in both clues, indicating the potential role of the lncRNA PVT1/miR186/KLF5 axis in the progression of CCA. Here, PVT1 is linked to cholangiocarcinoma.