In our former study, it was showed that siRNA-mediated SEMA4D gene silencing can increase chemosensitivity to 5-FU in CRC through several oncogenic pathways particularly by promoting apoptosis [25], but in this study, we aimed to investigate whether SEMA4D inhibition can directly be effective in tumor suppression. The gene discussed is SEMA4D; the disease is colorectal carcinoma.