Furthermore, the biomarkers of cardiac hypertrophy, the expressions of ANP, BNP, and MYH7 in rats from different groups, were detected by qRT-PCR, and according to the data, it is indicated that DOX induced the increase in the expression of the biomarkers of cardiac hypertrophy, which was enhanced by the overexpression of HMGB1 and inhibited by the knockdown of HMGB1 and treatment using DXZ (Figure 3J, P < 0.01). This evidence concerns the gene NPPB and cardiac hypertrophy.