Considering that fresh liver samples were limited to liver fibrosis/cirrhosis samples in the vicinity of liver cancer, we examined the hepatic BRD4 expression pattern using pathologic specimens from patients with liver fibrosis/cirrhosis with a variety of etiologies, including HBV, HCV, AIH, PBC, NASH, cholestasis, and overlap syndrome. This evidence concerns the gene BRD4 and overlapping connective tissue disease.