Sca1-ETV6-RUNX1 + Pax5-het B-ALL clustered together with B-ALL that originated as a result of Pax5 loss (Martin-Lorenzo et al., 2015), whereas Sca1-ETV6-RUNX1 + Kdm5cf/wt + Sca1-Cre B-ALL clustered with ETV6-ETV6–RUNX1 + Sca1-Cre B-ALL. The gene discussed is RUNX1; the disease is precursor B-cell acute lymphoblastic leukemia.