After neutrophils were depleted (Ndep) by anti-rat-neutrophil serum, hepatic neutrophil accumulation and infiltration in Ndep-DSS + SOS and Ndep-DSS + DILI groups were abolished, as demonstrated by IHC-MPO and flow cytometry (Supplementary Figures 10A–D). This evidence concerns the gene MPO and spondylo-ocular syndrome.