After being treated with rTsPmy, the CD3+CD4+ T cells expressing IL-17A and IFN-γ were significantly reduced in the colonic lamina propria mononuclear cells (LPMCs) of mice with DSS-induced colitis compared to group treated with PBS only (Figures 4A,B), indicating the colitis-related inflammatory Th1 and Th17 responses were inhibited by TsPmy treatment. This evidence concerns the gene CD4 and colitis.