Further investigation identified that rTsPmy actually stimulated thymus-derived Tregs (tTregs) expressed with the transcription factor Helios (Helios+) rather than the peripherally derived Tregs (pTregs) in the cLP of mice with DSS-induced colitis (Figure 5B); the former plays a more suppressive and regulatory role in the extreme inflammatory environment (Clough et al., 2020). The gene discussed is IKZF2; the disease is colitis.