However, the differences between these results and those obtained with the TLR9 silencing assay and p38/ERK inhibition experiments suggest that the positive regulatory effects associated with the p38 and ERK pathways were more important than the negative regulatory effects related to the AKT pathway for the TLR9-mediated innate immune response against Giardia infection in mouse macrophages. This evidence concerns the gene AKT1 and giardiasis.