Immunohistochemical staining showed that, compared to 14-week-old JFKWT/PyMTTG mice, the Hif-1α and Jfk protein levels were clearly up-regulated in the mammary tumors of the 16-week-old JFKWT/PyMTTG mice (Figure 3D). The gene discussed is HIF1A; the disease is breast cancer.