Notably, AML samples with CBFB mutation, mainly CBFB-MYH11 fusion gene mutation, were clustered together with those with mutation targeting DNMT3A (Figure 2B and Supplementary Figure 1), suggesting the possibility that mutations targeting CBFB, like those targeting DNMT3A, may impair DNA methylation process and reduce DNA methylation level. Here, MYH11 is linked to acute myeloid leukemia.