Mechanically, pathway analysis showed that PSMC5 regulated EMT of CRC cells, which was verified by the fact that decreased cell growth and invasion of shPSMC5 cells could be rescued by expression of Twist. Therefore, these results demonstrated that PSMC5 expression was a prognostic factor of CRC, and altered expression of PSMC5 in CRC cells had multiple functions on tumor cell behavior. This evidence concerns the gene TWIST1 and neoplasm.