However, METTL3 overexpression showed no effect on the colony formation of TMZ-resistant GBM cells, suggesting that a highly expressed and super-functional role of METTL3 in TMZ-resistant GBM cells, thus further overexpression of METTLE3 increased the total m6A methylated mRNAs, but did not enhance the cell proliferation ability of TMZ-resistant GBM cells. The gene discussed is METTL3; the disease is glioblastoma.