Interestingly, siRNA mediated DCLK1 depletion in 3 HNSCC cell lines (JHU-011 JHU-022 and JHU-029) substantially downregulated the expression levels of the NOTCH1 intracellular domain (NICD) and its downstream targets, such as HES1, HES5 and HEY1 (Figure 5A), confirming that NOTCH signaling was functionally inhibited. The gene discussed is HES1; the disease is head and neck squamous cell carcinoma.