Supplementary Figures 1C, D shows the average tumor volume of animals in different treatment groups from the starting till the end of the study. The results also demonstrate that curcumin fails to chemosensitize TS-deficient MDA-MB-231 cells to 5-FU therapy (Figures 5A-ii). IHC studies of TS and NF-κB in the tumor samples derived from animals bearing MDA-MB-231TS/MDA-MB-231TS- xenografts confirmed TS-dependent down-regulation of NF-κB (Figures 5Di-ii). This evidence concerns the gene TYMS and neoplasm.