The result of flow cytometry showed that PD-1 was significantly upregulated after transfection of SNHG3 overexpression plasmid (p<0.001), and ASF1B knockdown partially reversed the effect of lncRNA SNHG3 on PD-1 expression in HCC cell (p<0.001) (Figure 9H). The gene discussed is PDCD1; the disease is hepatocellular carcinoma.