NOS3 and endothelial dysfunction: Furthermore, methylglyoxal incubation significantly inhibited endothelium-dependent vasodilatation and decreased the expression of endothelial nitric oxide synthase (eNOS) in thoracic aorta of aged Wistar rats in vitro, while resveratrol treatment could improve methylglyoxal-induced endothelial dysfunction by increasing eNOS expression and activity [41].